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Journal of Fish Biology · 2010 · Vol. 76 · Issue 2 · Wiley
The amplified fragment‐length polymorphism (AFLP) technique was used to identify sex‐specific markers in bluegill sunfish. A total of 12 835 loci were produced by 256 primer combinations, of which nine (0·73‰) exhibited presumed sex‐associated amplifications in the pooled samples; however, none of which revealed sex specificity upon individual evaluation.