Summary Cyclophilins are ubiquitous proteins found in all domains of life, catalyzing peptidyl‐prolyl cis‐trans isomerization (PPIase activity) and functioning in diverse cellular processes. The filamentous insect pathogenic fungus, Beauveria bassiana , contains 11 cyclophilin genes whose roles were probed via individual gene knockouts, construction of over‐expression strains, and a simultaneous gene knockdown strategy using t...