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Journal: Journal of Applied Phycology ×Author: Li-Rui Liu ×Clear All Filters
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Journal of Applied Phycology · 2022 · Vol. 34 · Issue 3 · Springer
Genetic manipulation of Haematococcus pluvialis is difficult because of the lack of a stable and convenient transformation system. The pH124-EGFP-Ble vector containing ble as a selective gene and EGFP as a reporter gene was constructed and employed for effective transformation. H. pluvialis protoplasts were obtained by treating with cellulase and macerozeme. Then polyethylene glycol-mediated transformation was established by i...