Singapore grouper iridovirus (SGIV) is a major viral pathogen threatening grouper aquaculture. The lack of specific antiviral drugs underscores the urgent need to develop safe and effective control strategies. This study demonstrates that tannic acid (TA) exhibits significant anti‐SGIV activity in vitro. In grouper spleen (GS) cells, TA inhibited SGIV replication in a concentration‐dependent manner. Time‐of‐addition assays con...

(1) Background: Vaccination is crucial for Ctenopharyngodon idella, yet the impact of delivery methods on welfare and immunity requires investigation. (2) Methods: This study compared manual (HI-V) and automated (AI-V) injections of inactivated Aeromonas hydrophila vaccine. (3) Results: Both methods induced protective immunity, with AI-V achieving a slightly higher Relative Percent Survival (41.67%) than HI-V (37.84%). AI-V pr...

Singapore grouper iridovirus (SGIV) is a highly pathogenic virus that causes substantial economic losses in marine grouper aquaculture, and effective antiviral agents are currently lacking. In this study, the anti‐SGIV activity of epigallocatechin gallate (EGCG), a natural compound from green tea, was evaluated and its underlying mechanisms were investigated. Cytotoxicity assays showed that EGCG at concentrations up to 12.5 μg...