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This study optimised the immunisation protocol for rainbow trout fingerlings against infectious haematopoietic necrosis virus (IHNV). Fish were administered an experimental vaccine via intraperitoneal injection, consisting of formalin‐inactivated IHNV formulated with Montanide ISA 763A VG. A 2 × 2 factorial design was employed to compare two injection volumes (50 μL or 150 μL) and two immunisation regimens (single versus doubl...
Development of a Csy4-Processing TRV-Based CRISPR/Cas9 Genome Editing System in Nicotiana benthamianaNARA Subscribed
The CRISPR/Cas9 system is a site-specific genome editing tool that has been widely used in various plant species. The plant virus-based gRNA (guide RNA) delivery system, which differs from the typical Agrobacterium -mediated transformation, is an attractive method to facilitate the application of CRISPR/Cas9. The virally delivered gRNA is usually driven by heterologous plant U6 or viral promoters (e.g., pea early-browning viru...