Rapid depressurization and warming during recovery can trigger stress in deep-sea microbes and accelerate RNA degradation. We developed a remotely operated vehicle (ROV)-oriented multi-sequence microbial sampler for 2000 m sampling (20 MPa, 2 °C) that integrates in situ filtration with immediate RNAlater injection (an RNA stabilization reagent), collecting up to 12 samples per dive. A Dirichlet sampling–B-spline–SVM framework...