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The leopard coral grouper (Plectropomus leopardus), an economically important marine fish, is widely farmed in Hainan Province, China. However, intensive farming has led to frequent disease outbreaks, including viral nervous necrosis, vibriosis, and enteritis, with skin ulceration a widespread and significant concern. To identify the pathogen causing skin ulceration, we collected diseased fish from Hainan and conducted histopa...
Development and Evaluation of a Rapid Visualisation Detection Method for Ameson portunus Based on RPA ‐ LFDNARA Subscribed
The Ameson portunus is a prevalent pathogen affecting Portunus trituberculatus , which can infect P. trituberculatus and cause albinism, seriously damaging its economic value. Therefore, there is a pressing need for an efficient detection platform to rapidly and sensitively identify A. portunus . In this study, we have developed a method known as recombinase polymerase amplification combined with lateral flow dipstick (RPA‐LFD...
Development of Three Recombinase Polymerase Amplification Assays for the Rapid Visual Detection of Spiroplasma eriocheirisNARA Subscribed
Spiroplasma eriocheiris is a pathogen in the Chinese mitten crab Eriocheir sinensis and is associated with tremor disease. S. eriocheiris infects virtually all artificially bred crustaceans, resulting in significant economic losses to the aquaculture industry in China. Recombinase polymerase amplification (RPA) is considered a promising technology for the rapid and sensitive detection of disease. For the rapid detection of S....