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This study optimised the immunisation protocol for rainbow trout fingerlings against infectious haematopoietic necrosis virus (IHNV). Fish were administered an experimental vaccine via intraperitoneal injection, consisting of formalin‐inactivated IHNV formulated with Montanide ISA 763A VG. A 2 × 2 factorial design was employed to compare two injection volumes (50 μL or 150 μL) and two immunisation regimens (single versus doubl...