NARA Discovery
NARA Subscribed Scholarly Literature Discovery
Boolean search: climate AND coral · microplastic OR nanoplastic · plastic NOT freshwater · "marine heatwave"
Advanced Search
Topic searches article titles and abstracts. Starts a new search; publisher and year refine it.
Searches article titles and abstracts.
Accepts bare DOI, doi: prefix, or a doi.org URL.
Complete publisher name. Publisher imprints are grouped under their main publisher where applicable.
Exact match by full journal title or ISSN.
–
Refine Results Clear Filters
Collection
NARA Subscribed1
Publisher
Springer Nature1
Journal
Biotechnology Letters1
Publication Year
20221
Lorenzo Scutteri results 1 · Newest (Page 1/1, per page 25)
Export CSV Export RIS Export current results · up to 5,000 records
Author: Lorenzo Scutteri ×Clear All Filters
Search Results
High-yield production in Escherichia coli and convenient purification of a candidate vaccine against SARS-CoV-2NARA Subscribed
Biotechnology Letters · 2022 · Vol. 44 · Issue 11 · Springer
Giulia Maltoni; Lorenzo Scutteri; Francesca Mensitieri; Fabrizio Dal Piaz; Alejandro Hochkoeppler
Objectives The aim of the present work was to identify a time-saving, effective, and low-cost strategy to produce in Escherichia coli a protein chimera representing a fusion anti-SARS-CoV-2 candidate vaccine, consisting of immunogenic and antigenic moieties. Results We overexpressed in E. coli BL21(DE3) a synthetic gene coding for CRM197-RBD, and the target protein was detected in inclusion bodies. CRM197-RBD was solubilized w...
View Details View Full Text