Summary Dental unit waterlines (DUWL) support growth of a dense microbial population that includes pathogens and hypersensitivity‐inducing bacteria, such as Legionella spp. and non‐tuberculous mycobacteria (NTM). Dynamic dental instruments connected to DUWL generate aerosols in the work environment, which could allow waterborne pathogens to be aerosolized. The use of the real‐time quantitative polymerase chain reaction (qPCR)...