Sperm viability is one of the key in vitro parameters of sperm quality closely related to fertility. Typically, sperm viability is assessed by flow cytometry (FC) with propidium iodide (PI) as the fluorescent stain. However, as PI emits in the red spectrum, it can overlap with other commonly used fluorochromes. Utilizing a blue viability stain, such as DAPI, for semen evaluation would free the red channel, enabling the use of...