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Wiley1
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New Zealand Journal of Marine and Freshwater Research1
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Year: 2013 ×Author: M Devane ×Clear All Filters
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Recommendations for the processing and storage of water samples before polymerase chain reaction (PCR) analysisNARA Subscribed
New Zealand Journal of Marine and Freshwater Research · 2013 · Vol. 47 · Issue 4 · Wiley
BJ Gilpin; M Devane; F Nourozi; B Robson; P Scholes; S Lin
A range of options for the storage and partial processing of water samples before polymerase chain reaction (PCR) analysis were evaluated. In addition, temporal storage of extracted DNA at 4 °C was investigated. Filtering the water sample and then storing that filter at −20 °C for up to six months before DNA extraction was equivalent to extracting DNA immediately. Freezing a water sample resulted in an immediate 1 log loss of...
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