Standard information reporting helps to ensure that assay conditions and data are consistently reported and to facilitate inter-laboratory comparisons. Here, we present recommendations on minimum information for reporting on the TEER (trans-epithelial/endothelial electrical resistance) assay (MIRTA). The TEER assay is extensively used to evaluate the health of an epithelial/endothelial cell culture model and as an indicator of...

The solubility of iron (Fe) in seawater is greatly enhanced by complexation with organic ligands, predominantly occurring as humic substances (HS) in coastal areas. Mining exploitation is believed to change the natural biogeochemical cycle of Fe in coastal waters, even though its impacts on the physical and chemical speciation of the Fe fractions are not known. Here we show that dissolved and soluble Fe concentrations in coast...

Summary Microbial communities inhabit algae cell surfaces and produce a variety of compounds that can impact the fitness of the host. These interactions have been studied via culturing, single‐gene diversity and metagenomic read survey methods that are limited by culturing biases and fragmented genetic characterizations. Higher‐resolution frameworks are needed to resolve the physiological interactions within these algal–bacter...

A new method, based on the direct detection of iron‐humic substance (HS) species by cathodic stripping voltammetry (CSV), is used to determine the iron binding capacity and complex stability of fulvic acid (FA), humic acid (HA), and the natural HS in the seawater. The FA binds 16.7 ± 2.0 nmol iron (Fe) (mg FA)−1, whereas the HA and the marine HS bind 32 ± 2.2 nmol Fe (mg FA) ‐1 . The complex stabilities are (log K’ Fe’HS value...

We determined the chemical speciation of lead and copper in remote mountain and high‐latitude lakes at different times of the year, providing background data for regions in Europe least affected by anthropogenic effects. The lakes are characterized by low ionic strength, clear waters, and oligotrophic conditions; are fed predominantly by atmospheric precipitation; and are ice‐covered during a large part of the year. Lead—and c...

Previous work showed that insecticide resistance in Drosophila melanogaster is correlated with the insertion of an Accord ‐like element into the 5′ region of the cytochrome P450 gene, Cyp6g1 . Here, we study the distribution of the Accord ‐like element in 673 recently collected D. melanogaster lines from 34 world‐wide populations. We also examine the extent of microsatellite variability along a 180‐kilobase (kb) genomic region...

Marine microalgae ( Emiliania huxleyi ) were grown in seawater enriched only with nitrogen and phosphorus, without control of free metal concentrations using synthetic chelators. Complexing ligands and thiol compounds were determined by cathodic stripping volt–ammetry. Copper was added to these cultures, and ligands were produced in response to the copper addition. Parallel measurements of thiols showed that glutathione and ot...