M. Rhode results 116
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Use of translational fusions to express functional Klebsiella oxytoca dinitrogenase reductase in plant mitochondriaNARA Subscribed
Engineering crop plants with the biological nitrogen fixation pathway is a longstanding goal of modern agriculture. Dinitrogenase reductase (NifH) is a critical component of the biological nitrogen fixation pathway, with multiple roles in metal cofactor assembly and catalysis. This enzyme must be folded correctly as a soluble homodimer and loaded with the [4Fe-4S] metallocluster for function. Previous studies have found that K...
Uncovering correlates of decline and critical refuges for a threatened terrestrial mammalNARA Subscribed
Globally, the geographic ranges of numerous species are contracting. Identifying spatiotemporal patterns of threat impact can illuminate why species decline in some parts of their range but persist in others. We developed a correlative approach to identify species’ response thresholds and locate ecological refuge areas associated with persistence to inform conservation planning and management. We integrated changes in realized...
Recent fissure-fed eruptions from the Svartsengi Volcanic System have significantly impacted Iceland’s Reykjanes Peninsula. With eruptions likely to continue intermittently for decades to centuries, exploring the controls on the locations of fissure-fed eruptions is important for hazard assessment and risk mitigation. This study maps eruptive fissures from six eruptions from December 2023 to September 2024 using satellite and...
Advancing provitamin A biofortification in sorghum: from allele mining and marker development to prebreeding and breedingNARA Subscribed
Key message Allele mining for provitamin A biofortification in sorghum is advanced with new pre-bred germplasm and markers that are trait-predictive in diverse germplasm and breeding programs. Abstract Provitamin A biofortification of the staple cereal crop sorghum ( Sorghum bicolor [L.] Moench) could reduce vitamin A deficiency in low-income countries of sub-Saharan Africa. To advance mining of alleles that confer high provit...
Neonicotinoids (NEOs) are the most widely used class of insecticides worldwide. Consequently, human exposure occurs through multiple pathways, including dietary intake and environmental contact. Previous analytical methods have primarily focused on parent compounds, often excluding key metabolites due to matrix effects and poor quantification in complex biological samples. This gap severely limits the ability to accurately ass...
Utilizing Exhaled Breath and Skin Microbiomes in Assessments of At‐Risk Killer Whales ( Orcinus orca )NARA Subscribed
For at‐risk populations of killer whales ( Orcinus orca ), biological samples can offer information about individual animal status. We hypothesized that low‐yield samples could provide sufficient material for microbiome analysis. We collected and analyzed, with amplicon metabarcoding, breath samples from managed care and free‐ranging killer whales (8 and 14 animals, respectively) and epidermal tissue from free‐ranging killer w...
Effects of plant growth regulators on post-cryopreservation recovery and respiratory rate of Green Kangaroo Paw shoot tipsNARA Subscribed
Plant growth regulators can have a significant impact on regeneration and regrowth of shoot tips after cryopreservation. This study investigated four cytokinins, two auxins and gibberellic acid at three different concentrations to assess their effect on the quality of regenerating shoot tips of the cryotolerant species Green Kangaroo Paw ( Anigozanthos viridis ) over a 400 h (approximately 2 weeks) recovery period. Shoot tip w...
Individual differences in behaviour and behavioural plasticity have been extensively studied in a variety of animals across the phylogenetic spectrum. Amphiprion species bring distinct insight into the topic because of their unique life history, mating system, and extraordinary degree of behavioural plasticity associated with protandrous (male‐to‐female) sex change. Several laboratory studies have begun characterizing individu...
Immunogenetics, sylvatic plague and its vectors: insights from the pathogen reservoir Mastomys natalensis in TanzaniaNARA Subscribed
Yersinia pestis is a historically important vector-borne pathogen causing plague in humans and other mammals. Contemporary zoonotic infections with Y. pestis still occur in sub-Saharan Africa, including Tanzania and Madagascar, but receive relatively little attention. Thus, the role of wildlife reservoirs in maintaining sylvatic plague and spillover risks to humans is largely unknown. The multimammate rodent Mastomys natalensi...