The use of photosynthetic biohybrids to drive redox reactions in vitro is a promising strategy due to the natural abundance and high quantum efficiency of the bio-components. Here, we present different tools for the fabrication of photosystem I-based biohybrid devices by using redox-active polymers, 3D-structured electrodes as well as additional light-harvesting antennae modules.

The assembly of large, multi-cofactor membrane protein complexes like photosystem II (PSII) requires a high level of coordination. The process is facilitated by a large network of auxiliary proteins that bind transiently to unassembled subunits, preassembled modules or intermediate states of PSII, which are comprised of a subset of subunits. However, analysis of these immature, partially assembled PSII complexes is hampered by...

The highly optimized natural process of oxygenic photosynthesis leads to the formation of redox equivalents, such as NADPH, that can be used to fuel heterologous biotransformations in phototrophic microorganisms. We investigated the reduction of 2-methylmaleimide by the ene-reductase YqjM in the cyanobacterium Synechocystis sp. PCC 6803 and doubled the productivity of the cells by inactivating flavodiironproteins (FDPs) as com...