Monitoring biodiversity patterns and their changes in Arctic coastal ecosystems is critical under ongoing climate change. However, common current approaches require high effort and expertise and this in turn limits the spatial and temporal scale of these monitoring efforts. Here, we investigated both the fish and the marine invertebrate communities across Svalbard using a multi-marker environmental DNA metabarcoding approach....

Macroinvertebrates are widely used in freshwater biomonitoring, but current methods are labor-intensive and require taxonomic expertise. DNA metabarcoding offers a potential solution, though results vary per applied sampling protocol. Here, we compare several protocols using macroinvertebrate samples from a Dutch peatland. Live-sorted specimens were (i) identified morphologically followed by (ii) non-destructive (soft-lysis) a...