Background Mycobacterium bovis BCG is widely used as a vaccine vector due to its safety, genetic stability, and immunomodulatory properties. However, current expression systems for BCG transformation rely on antibiotic resistance genes, raising biosafety concerns. Therefore, alternative strategies—such as the use of auxotrophic strains—are highly desirable. Our group previously employed BioBrick™ technology to construct the pU...

The insidious onset and progression of sarcopenia make it vital to understand the early skeletal muscle changes and explore therapies to slow its progression. This study explored the gastrocnemius remodeling at an early stage of aging (14 months of age) and the effects of lifelong aerobic exercise. For that, 2-month-old male Wistar rats underwent a 12-month treadmill exercise program. Sedentary age-matched, young sedentary, an...

Cetaceans are sentinels of marine ecosystem health and are increasingly exposed to protozoal pathogens. This study investigated the occurrence and molecular identity of Sarcocystidae protozoa in 159 stranded cetaceans representing 21 species along the Brazilian coast. Molecular analysis based on PCR amplification and sequencing of the sarcocystid internal transcribed spacer 1 (ITS1) region revealed infections in 14 individuals...

Megninia ginglymura (Mégnin, 1877) (Acariformes: Analgoidea: Analgidae) is a common feather mite of laying hens and a potential contributor to plumage damage and reduced productivity, yet its population dynamics and microhabitat use remain insufficiently characterized under modern commercial conditions. We conducted a year-long assessment of M. ginglymura abundance and intra-host distribution across three major production syst...

Zoonotic parasites are an increasingly recognised challenge for seafood safety. Anisakid nematodes currently represent the most significant fish‐borne parasitic threat in Atlantic wild fisheries. Recent regional surveys and global syntheses suggest significant increases in the prevalence and intensity of anisakid infections across multiple commercially important fish species, raising concerns for public health, marketability a...

Human T-lymphotropic virus type 1 (HTLV-1) infection is associated with a broad spectrum of neurological manifestations, including conditions that may precede the development of HTLV-1–associated myelopathy/tropical spastic paraparesis (HAM/TSP). This analytical, descriptive cross-sectional study aimed to estimate the prevalence of HTLV-1 infection and describe associated sociodemographic, socioeconomic, behavioral, and clinic...

Background Giardiasis remains a globally prevalent neglected parasitic disease, and limitations associated with conventional therapies highlight the need for alternative and complementary strategies. In this context, microalgae biocompounds have emerged as promising sources of antiparasitic agents. Objective This study aims to evaluate the in vitro preliminary screening activity (vital dye exclusion). Methods Aqueous extracts...

Codium tomentosum is currently cultivated through vegetative propagation (cuttings) in tumble culture systems targeting niche markets. To scale up and expand its aquaculture production, optimization of propagation and culture methods is key. In Asia, artificial seed production via regeneration of isolated utricles and medullary filaments has already been established for the commercial production of Codium fragile . Our prelimi...