During the analysis of suspensions of PI-stained nuclei from stem, adventitious roots, and leaves of in vitro germinated juvenile D. majalis plants, a range of one to three consecutive rounds of strict partial genome duplications could be discovered. Repeated cycles of under-replications were found again when axenically grown material was moved from solid nutrient media to potting soil, where it further maturated within and ou...

The orchid species Dactylorhiza majalis is endangered by continuing habitat destruction and fragmentation. This requires more detailed information with respect to its sexual reproduction, which is especially relevant for Germany, where from 10 % to 30 % of the world-wide remaining populations grow. In the present study, we determined both the numbers of growing and flowering individuals per stand with regard to D. majalis at 1...