Groundwater ecosystems sustain drinking water supplies and play key roles for global biogeochemical cycles. Their functions are largely conveyed by microorganisms; so to understand and predict their response to global change, functional insights into groundwater microbiomes are essential. By combining metabolic labeling and omics methods to mechanistically trace microbial functions, we aim to reveal the role of non-phototrophi...

Purpose Currently, body weight-based dosing of rifampicin is recommended. But lately, fat-free mass (FFM) was reported to be superior to body weight (BW). The present evaluation aimed to assess the influence of body mass-related covariates on rifampicin’s pharmacokinetics (PK) parameters in more detail using non-linear mixed effects modeling (NLMEM). Methods Twenty-four healthy Caucasian volunteers were enrolled in a bioequiva...

Summary The release of abiotic methane from marine seeps into the atmosphere is a major source of this potent greenhouse gas. Methanotrophic microorganisms in methane seeps use methane as carbon and energy source, thus significantly mitigating global methane emissions. Here, we investigated microbial methane oxidation at the sediment–water interface of a shallow marine methane seep. Metagenomics and metaproteomics, combined wi...

Summary Microbial activity is key in understanding the contribution of microbial communities to ecosystem functions. Metabolic labelling with heavy water (D 2 O) leads to the formation of carbon–deuterium bonds in active microorganisms. We illustrated how D 2 O labelling allows monitoring of metabolic activity combined with a functional characterization of active populations in complex microbial communities. First, we demonstr...

Summary Nitrogen is a key limiting resource for biomass production in the marine environment. Methylated amines, released from the degradation of osmolytes, could provide a nitrogen source for marine microbes. Thus far, studies in aquatic habitats on the utilization of methylamine, the simplest methylated amine, have mainly focussed on the fate of the carbon from this compound. Various groups of methylotrophs, microorganisms t...

Summary In constructed wetlands, organic pollutants are mainly degraded via microbial processes. Helophytes, plants that are commonly used in these systems, provide oxygen and root exudates to the rhizosphere, stimulating microbial degradation. While the treatment performance of constructed wetlands can be remarkable, a mechanistic understanding of microbial degradation processes in the rhizosphere is still limited. We investi...

Summary The xox F gene, encoding a pyrroloquinoline quinone‐dependent methanol dehydrogenase, is found in all known proteobacterial methylotrophs. In several newly discovered methylotrophs, XoxF is the active methanol dehydrogenase, catalysing the oxidation of methanol to formaldehyde. Apart from that, its potential role in methylotrophy and carbon cycling is unknown. So far, the diversity of xox F in the environment has recei...

Summary A variety of culture‐independent techniques have been developed that can be used in conjunction with culture‐dependent physiological and metabolic studies of key microbial organisms in order to better understand how the activity of natural populations influences and regulates all major biogeochemical cycles. In this study, we combined deoxyribonucleic acid‐stable isotope probing (DNA‐SIP) with metagenomics and metaprot...