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Summary During a field experiment applying broiler manure for fertilization of agricultural land, we detected viable Clostridioides (also known as Clostridium ) difficile in broiler faeces, manure, dust and fertilized soil. A large diversity of toxigenic C . difficile isolates was recovered, including PCR ribotypes common from human disease. Genomic relatedness of C . difficile isolates from dust and from soil, recovered more...
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