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Springer Nature1
Journal
Stem Cell Reviews and Reports1
Publication Year
20241
Max Mackevicius results 1 · Newest (Page 1/1, per page 25)
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Single cell, Label free Characterisation of Human Mesenchymal Stromal cell Stemness and Future Growth Potential by Autofluorescence Multispectral ImagingNARA Subscribed
Stem Cell Reviews and Reports · 2024 · Vol. 20 · Issue 8 · Springer
Jared M. Campbell; Abbas Habibalahi; Adnan Agha; Shannon Handley; Aline Knab; Xiaohu Xu; Akanksha Bhargava; Zhilin Lei; Max Mackevicius; Yuan Tian; Saabah B. Mahbub; Ayad G. Anwer; Stan Gronthos; Sharon Paton; Shane T. Grey; Lindsay Wu; Robert B. Gilchrist; Ewa M. Goldys
Aim To use autofluorescence multispectral imaging (AFMI) to develop a non-invasive assay for the in-depth characterisation of human bone marrow derived mesenchymal stromal cells (hBM-MSCs). Methods hBM-MSCs were imaged by AFMI on gridded dishes, stained for endpoints of interest (STRO-1 positivity, alkaline phosphatase, beta galactosidase, DNA content) then relocated and results correlated. Intensity, texture and morphological...
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