The analysis of environmental DNA (eDNA) has revolutionized biodiversity assessments in aquatic ecosystems, enabling non‐invasive monitoring of fish communities across diverse regions. However, the global comparability of these eDNA datasets remains ambiguous due to heterogeneous sampling protocols and bioinformatic workflows across studies, making it difficult to assess how robust and comparable the biodiversity patterns infe...

Aquatic and terrestrial communities often co‐occur at close distances, enabling biotic interactions across ecosystem boundaries. While such interactions in natural habitats contribute to complex, coupled food webs, their dynamics in engineered and fragmented urban habitats are hardly known. Using environmental DNA metabarcoding and a metaweb approach, we examined food web structure at 54 paired aquatic‐terrestrial sites along...

Monitoring of terrestrial and aquatic species assemblages at large spatial scales based on environmental DNA (eDNA) has the potential to enable evidence-based environmental policymaking. The spatial coverage of eDNA-based studies varies substantially, and the ability of eDNA metabarcoding to capture regional biodiversity remains to be assessed; thus, questions about best practices in the sampling design of entire landscapes re...