Circular RNAs (circRNAs) were first identified approximately 50 years ago in pathogenic viroids as single-stranded, covalently closed RNA molecules. Initially considered by-products of splicing, circRNAs are now recognised as an important class of regulatory RNAs involved in microRNA sponging, RNA–protein interactions, and cellular pathways. Their closed-loop structure, generated through backsplicing, confers resistance to exo...

Alzheimer’s disease (AD) is a neurodegenerative disorder that progressively deteriorates a person’s memory, as well as their ability to think and move. It has been reported to be the most common cause of dementia. Alterations in gene expression have been increasingly recognised as key contributors to the onset and progression of AD, driving interest in transcriptomic approaches to better understand the disease at a molecular l...

Recent improvements in the accuracy of long-read sequencing (LRS) technologies have expanded the scope for novel transcriptional isoform discovery. Additionally, these advancements have improved the precision of transcript quantification, enabling a more accurate reconstruction of complex splicing patterns and transcriptomes. Thus, this project aims to take advantage of these analytical developments for the discovery and analy...

Circular RNAs (circRNAs) are a subclass of non-coding RNAs which have demonstrated potential as biomarkers for Alzheimer’s disease (AD). In this study, we conducted a comprehensive exploration of the circRNA transcriptome within AD brain tissues. Specifically, we assessed circRNA expression patterns in the dorsolateral prefrontal cortex collected from nine AD-afflicted individuals and eight healthy controls. Utilising two circ...