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Detection of koi herpesvirus in common carp, Cyprinus carpio L., by loop‐mediated isothermal amplificationNARA Subscribed
Loop‐mediated isothermal amplification (LAMP) is a novel method that amplifies DNA with high specificity and rapidity under isothermal conditions. In this study, using the LAMP method, a protocol for koi herpes virus (KHV) detection in common carp was designed. A set of four primers, two inner and two outer, were designed based on the sequence of the thymidine kinase ( tk ) gene of KHV. Time and temperature conditions for dete...
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