Neil M Price results 27
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The influence of light on the availability of photoreactive and photostable iron( III )–ligand complexes to diatomsNARA Subscribed
Uptake of iron (Fe) by eukaryotic phytoplankton is suggested to proceed by a reductive pathway in which Fe(III) bound to inorganic and organic ligands is reduced prior to Fe internalization. Photochemical reduction of the Fe–ligand complexes can increase Fe bioavailability, but the role of light in regulating physiological processes related to Fe acquisition is not well known. Here, we report how model Fe–ligand complexes diff...
Enhanced vertical stratification brought about by warming of the ocean surface is expected to reduce vertical circulation and nutrient input with knock-on effects for phytoplankton. Increased nutrient limitation is one predicted outcome, but how that will impact phytoplankton is uncertain because we do not know how they will adapt. We used copper (Cu) as a model catalytic nutrient to explore the adaptive response of an oceanic...
Transcriptomes of an oceanic diatom reveal the initial and final stages of acclimation to copper deficiencyNARA Subscribed
Summary Copper (Cu) concentration is greatly reduced in the open sea so that phytoplankton must adjust their uptake systems and acclimate to sustain growth. Acclimation to low Cu involves changes to the photosynthetic apparatus and specific biochemical reactions that use Cu, but little is known how Cu affects cellular metabolic networks. Here we report results of whole transcriptome analysis of a plastocyanin‐containing diatom...
Common, low-frequency, rare, and ultra-rare coding variants contribute to COVID-19 severityNARA Subscribed
The combined impact of common and rare exonic variants in COVID-19 host genetics is currently insufficiently understood. Here, common and rare variants from whole-exome sequencing data of about 4000 SARS-CoV-2-positive individuals were used to define an interpretable machine-learning model for predicting COVID-19 severity. First, variants were converted into separate sets of Boolean features, depending on the absence or the pr...
With the accelerating pace of global change, it is imperative that we obtain rapid inventories of the status and distribution of wildlife for ecological inferences and conservation planning. To address this challenge, we launched the SNAPSHOT USA project, a collaborative survey of terrestrial wildlife populations using camera traps across the United States. For our first annual survey, we compiled data across all 50 states dur...
Copper(II) is reduced to Cu(I) extracellularly by marine and freshwater phytoplankton, but its biological significance is not firmly established. We studied the relationship between Cu(II) reduction and uptake in Thalassiosira oceanica , a diatom that was recently shown to possess functional copper uptake transporters (CTRs) that take up Cu(I). Inorganic and organic complexes of Cu(II) were reduced directly by reactions at the...
Functional CTR‐type Cu(I) transporters in an oceanic diatomNARA Subscribed
Summary Copper concentration is so low in some remote parts of the sea it limits phytoplankton growth, but may be high enough in coastal and estuarine regions to be toxic. Acclimation to variations in Cu concentration thus requires a tightly regulated Cu transport system to help maintain Cu homeostasis. In marine species, the molecular mechanisms of Cu transport are not known. We studied Cu‐responsive genes and uptake in Thala...
Identification of an iron permease, cFTR1, in cyanobacteria involved in the iron reduction/re‐oxidation uptake pathwayNARA Subscribed
Summary Cyanobacteria are globally important primary producers and abundant in many iron‐limited aquatic environments. The ways in which they take up iron are largely unknown, but reduction of Fe 3+ is an important step in the process. Here we report a special iron permease in Synechocystis , cFTR1, that is required for Fe 3+ uptake following Fe 2+ re‐oxidation. The expression of cFTR1 is induced by iron starvation, and a muta...
Enzymatic modification of schizophyllanNARA Subscribed
Poster abstractsNARA Subscribed
We have discovered that coastal and oceanic diatoms require more manganese (Mn) to grow in iron (Fe) ‐deficient than in Fe‐sufficient seawater. At low inorganic concentrations, like those of the open sea, Fe and Mn can thus colimit Thalassiosira pseudonana and T. oceanica so that maximum rates of cell division are achieved only when both resources are added simultaneously to cultures. Colimited diatoms amended with either Fe o...
Pathogenic gram‐negative bacteria use specific receptors to transport ferric siderophore complexes across their outer membrane during iron (Fe)‐limited growth. Receptors such as these have not yet been characterized in oceanic heterotrophic bacteria. We examined four species of γ‐proteobacteria for the presence of Fe‐siderophore receptors with the use of a nondenaturing polyacrylamide gel electrophoresis binding assay and the...
Water column interleaving: A new physical mechanism determining protist communities and bacterial statesNARA Subscribed
During a spring‐summer bloom in a large Arctic polynya, vertically distinct protist communities (phytoplankton and protozoa) occurred within layers caused by interleaving and entrainment of different water masses. We developed a ternary community distance index to quantify the variability in protist community structure in these heterogeneous surface layers. This index was highly correlated ( r = 0.984, n = 6) with the extent o...
Iron uptake and physiological response of phytoplankton during a mesoscale Southern Ocean iron enrichmentNARA Subscribed
Iron supply is thought to regulate primary production in high nitrate, low chlorophyll (HNLC) regions of the sea in both the past and the present. A critical aspect of this relationship is acquisition of iron (Fe) by phytoplankton, which occurs through a complex series of extracellular reactions that are influenced by Fe chemistry and speciation. During the first in situ mesoscale Fe‐enrichment experiment in the Southern Ocean...
Three species of photosynthetic flagellates capable of phagotrophy (mixotrophic species) were tested for their abilities to use inorganic iron colloids for growth. Ochromonas sp., Chrysochromulina ericina (a coastal strain), and C. ericina (an oceanic strain) were grown in iron‐free seawater supplemented with 1 µM Fe‐ferrihydrite (amorphous hydrous ferric oxide), magnetite (Fe 3 O 4 )/maghemite ( gamma‐Fe 2 O 3 ), hematite ( a...