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The Asia region harbors exceptional chondrichthyan (shark, ray, and chimaera) diversity but faces intense fishing pressure. The Important Shark and Ray Areas (ISRA) process provides a collaborative, evidence-based framework to identify critical habitats and inform spatial management. We assessed ISRAs across the Bay of Bengal, Southeast Asia, and the Northwest Pacific to characterize their extent, ecological significance, and...
Haploid embryogenic cultures of maize (Zea mays L.): stability, doubling efficiency, and use in gene editingNARA Subscribed
Embryogenic cultures were initiated from haploid and diploid seedlings of maize inbred LH244 and monitored for ploidy stability by flow cytometry over the course of six mo. Both culture types were transformed with three different constructs using Agrobacterium tumefaciens to compare transformation and gene editing frequencies. Paromomycin resistant cell lines were split prior to plant regeneration, and half of the tissue was t...
Peptoniphilus genitalis sp. nov. and Mobiluncus massiliensis sp. nov.: Novel Bacteria Isolated from the Vaginal MicrobiomeNARA Subscribed
The strains Marseille-Q7072 T (= CSUR Q7072 T = CECT 30604 T ) and Marseille-Q7826 T (= CSUR Q7826 T = CECT 30727 T ) were isolated from vaginal samples. As MALDI-TOF mass spectrometry failed to identify them, their genomes were directly sequenced to determine their taxogenomic identities. Both strains are anaerobic without any oxidase and catalase activity. C 16:0 is the most abundant fatty acid for both strains. Strain Marse...
Culturomics reveals a hidden world of vaginal microbiota with the isolation of 206 bacteria from a single vaginal sampleNARA Subscribed
The composition of the vaginal microbiota is known to be influenced by various factors and to be associated with several disorders affecting women’s health. Although metagenomics is currently a widely used method for studying the human microbiota, it has certain limitations, such as a lack of information on bacterial viability. It is therefore important to use culture-based methods such as culturomics. Here, we used 35 differe...
Cellulomonas endometrii sp. nov.: a novel bacterium isolated from the endometrial microbiotaNARA Subscribed
An isolate of a bacterium recovered from an endometrial biopsy failed to be identified by MALDI-TOF mass spectrometry and was subjected to 16S rRNA sequencing. The obtained sequence was compared by BLASTn against the NCBI database, which revealed that the most closely related species was Cellulomonas hominis and Cellulomonas pakistanensis , with 98.85% and 98.45% identity, respectively. Phenotypic characterisation and genome s...
Two strains, designated as Marseille-P2918 T and Marseille-P3646 T , were isolated from a 14-week-old Senegalese girl using culturomics: Urmitella timonensis strain Marseille-P2918 T (= CSUR P2918, = DSM 103634) and Marasmitruncus massiliensis strain Marseille-P3646 T (= CSUR P3646, = CCUG72353). Both strains were rod-shaped, anaerobic, spore forming motile bacteria. The 16S rRNA gene sequences of strains Marseille-P2918 T (LT...
Strains Marseille-P3761 and Marseille-P3195 are representatives of two bacterial species isolated from human specimens. Strain Marseille-P3761 was isolated from the stool of a healthy volunteer, while strain Marseille-P3915 was cultivated from the urine of a kidney transplant recipient. Both strains are anaerobic Gram-positive coccoid bacteria. Both are catalase-negative and oxidase-negative and grow optimally at 37 °C in anae...
Common, low-frequency, rare, and ultra-rare coding variants contribute to COVID-19 severityNARA Subscribed
The combined impact of common and rare exonic variants in COVID-19 host genetics is currently insufficiently understood. Here, common and rare variants from whole-exome sequencing data of about 4000 SARS-CoV-2-positive individuals were used to define an interpretable machine-learning model for predicting COVID-19 severity. First, variants were converted into separate sets of Boolean features, depending on the absence or the pr...
Modeling marine pelagic fish species spatiotemporal distributions utilizing a maximum entropy approachNARA Subscribed
Understanding how ocean conditions influence fish distributions is critical for elucidating the role of climate in ecosystem change and forecasting how fish may be distributed in the future. Traditional species distribution models are often applied to scientific‐survey data, which include species presence‐absence information, to predict distributions. Maximum entropy (MaxEnt) models are promising tools as they can be applied t...