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Haploid embryogenic cultures of maize (Zea mays L.): stability, doubling efficiency, and use in gene editingNARA Subscribed
Embryogenic cultures were initiated from haploid and diploid seedlings of maize inbred LH244 and monitored for ploidy stability by flow cytometry over the course of six mo. Both culture types were transformed with three different constructs using Agrobacterium tumefaciens to compare transformation and gene editing frequencies. Paromomycin resistant cell lines were split prior to plant regeneration, and half of the tissue was t...
A low oxygen threshold for physiological responses and trace metal quotas of Prochlorococcus and SynechococcusNARA Subscribed
Continued human activity is expected to accelerate ocean deoxygenation, leading to the expansion and shallowing of oxygen‐deficient zones (ODZs). This decline in oxygen may impact both phytoplankton growth and trace metal uptake. We conducted culture experiments with Prochlorococcus MIT9313 and Synechococcus XM‐24, two numerically dominant genera of marine picocyanobacteria, under varying O 2 levels (36–242 μ M). These experim...
Motivation Here, we make available a second version of the BioTIME database, which compiles records of abundance estimates for species in sample events of ecological assemblages through time. The updated version expands version 1.0 of the database by doubling the number of studies and includes substantial additional curation to the taxonomic accuracy of the records, as well as the metadata. Moreover, we now provide an R packag...
Monooxygenase (MO) enzymes are responsible for the oxidation of hydrocarbons and other compounds in the carbon and nitrogen cycles, are important for the biodegradation of pollutants and can act as biocatalysts for chemical manufacture. The soluble di‐iron monooxygenases (SDIMOs) are of interest due to their broad substrate range, high enantioselectivity and ability to oxidise inert substrates such as methane. Here, we re‐exam...