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Tilapia Lake Virus (TiLV) is a significant threat to global tilapia aquaculture, highlighting the need for rapid and accurate diagnostic methods to manage outbreaks and minimise economic losses. This study presents the development and partial validation of a one‐pot assay integrating RT‐LAMP with the CRISPR/Cas12b system for sensitive and specific TiLV detection. This assay amplifies viral RNA using RT‐LAMP, while CRISPR/Cas12...
Upwelling of hypolimnetic water in lakes is important for littoral food webs as it enhances production through nutrient subsidies to surface waters. The aim of this study was to understand the role of hypolimnetic upwelling on littoral nutrient supply and diet of a freshwater unionid mussel, kākahi ( Echyridella menziesii) in an oligotrophic lake using stable isotopes (δ 15 N) as a tracer of hypolimnetic nitrogen. Upwelling ev...
Larval Arctic cod ( Boreogadus saida ) exhibit stronger developmental and physiological responses to temperature than to elevated pCO 2NARA Subscribed
High‐latitude ecosystems are simultaneously warming and acidifying under ongoing climate change. Arctic cod ( Boreogadus saida ) are a key species in the Arctic Ocean and have demonstrated sensitivity to ocean warming and acidification as adults and embryos, but their larval sensitivity to the combined stressors is unknown. In a laboratory multi‐stressor experiment, larval Arctic cod were exposed to a combination of three temp...
Cowpea ( Vigna unguiculata (L.) Walp.) is the most important grain legume in sub-Saharan Africa where it provides a significant source of human nutrition and animal fodder to millions of low-input farmers. While adapted to a wide range of soils and generally considered climate resilient due to its drought and heat tolerance, cowpea yields are suboptimal throughout its main growing region because of numerous abiotic and biotic...
Nonequilibrium spherulitic magnetite in the Ryugu samplesNARA Subscribed
Offshore platforms, subsea pipelines, wells and related fixed structures supporting the oil and gas (O&G) industry are prevalent in oceans across the globe, with many approaching the end of their operational life and requiring decommissioning. Although structures can possess high ecological diversity and productivity, information on how they interact with broader ecological processes remains unclear. Here, we review the curren...
A sharp floristic discontinuity revealed by the biogeographic regionalization of African savannasNARA Subscribed
Aim In tropical Africa, savannas cover huge areas, have high plant species richness and are considered as a major natural resource for most countries. There is, however, little information available on their floristics and biogeography at the continental scale, despite the importance of such information for our understanding of the drivers of species diversity at various scales and for effective conservation and management. He...
Design standards for experimental and field studies to evaluate diagnostic accuracy of tests for infectious diseases in aquatic animalsNARA Subscribed
Design and reporting quality of diagnostic accuracy studies ( DAS ) are important metrics for assessing utility of tests used in animal and human health. Following standards for designing DAS will assist in appropriate test selection for specific testing purposes and minimize the risk of reporting biased sensitivity and specificity estimates. To examine the benefits of recommending standards, design information from published...
Biomarkers of Acute Stroke Etiology (BASE) Study MethodologyNARA Subscribed