The stomach of adult shi drum Umbrina cirrosa was investigated using a battery of nine horseradish peroxidase‐conjugated lectins combined with enzymatic treatment, in order to distinguish glycoconjugate sugar residues. Epithelial cells showed the presence of galactosyl(β1→4)N‐acetylglucosamine, mannose, N‐acetylgalactosamine, N‐acetylglucosamine, fucose and sialic acid‐galactosyl(β1→3)N‐acetylgalactosamine residues. Gastric pi...

The saccharide composition of surface and secretion glycoconjugates in the oesophagus of Umbrina cirrosa was examined by means of lectin histochemistry. Mucous cells showed the presence of N‐acetylgalactosamine, N‐acetylglucosamine and sialic acid linked to the dimer galactosyl(β1→3) N‐acetylgalactosamine. Columnar epithelial cells had a positive reaction with almost all the lectins employed, located in the supranuclear region...

Goblet cells in the intestine of shi drum Umbrina cirrosa showed the presence of glycoconjugates particularly rich in fucose and N‐acetylglucosamine residues. They displayed also sialic acid linked to galactosyl(β1→3)N‐acetylgalactosamine and to galactosyl(β1→4)N‐acetylglucosamine. All the nine horseradish peroxidase‐conjugated lectins employed with the only exception of GSA II marked the enterocytes supranuclear region and th...

The aim of this work is to elucidate the glycoconjugate composition of the secretory products of the oesophageal mucous cells in a tilapine polyhybrid. Lectin histochemistry gave evidence of the presence of β‐galactose, α‐N‐acetylgalactosamine and sialic acid residues in the terminal position. The majority of sialic acid belongs to short side chains; a few sialic acid residues are acetylated at the C 7 and/or C 8 and/or C 9 le...

The glycoconjugates secreted by the anterior and posterior intestinal segments of Tilapia spp. were characterized by means of both conventional histochemical procedures (PAS, AB, AB‐PAS, LID, HID) and a battery of 12 horseradish peroxidase labelled lectins. Some sections were treated with glycolytic enzymes and KOH before conventional and lectin stainings. The large majority of the mucopolysaccharides secreted by the goblet ce...