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Environmental Microbiology1
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Year: 2001 ×Author: Pascal Simonet ×Clear All Filters
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Quantification of bacterial subgroups in soil: comparison of DNA extracted directly from soil or from cells previously released by density gradient centrifugationNARA Subscribed
Environmental Microbiology · 2001 · Vol. 3 · Issue 7 · Wiley
Sophie Courtois; Åsa Frostegård; Pernilla Göransson; Geraldine Depret; Pascale Jeannin; Pascal Simonet
All molecular analyses of soil bacterial diversity are based on the extraction of a representative fraction of cellular DNA. Methods of DNA extraction for this purpose are divided into two categories: those in which cells are lysed within the soil (direct extraction) and those in which cells are first removed from soil (cell extraction) and then lysed. The purpose of this study was to compare a method of direct extraction with...
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