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Cell and Tissue Research · 2026 · Vol. 404 · Issue 2 · Springer
Proximal tubule in vitro systems are important tools in pre-clinical drug development, providing insights into drug disposition and safety. Cells are often cultured in medium containing fetal calf serum (FCS); however, FCS addition is not physiologically relevant for cells of human origin. Here, in vitro cultures of human kidney proximal tubule cells were transitioned to FCS-free medium. The impact of FCS-free medium on matura...