The cultivation and consumption of blackberry and blueberry are expanding, with micropropagation emerging as the dominant propagation method. In the present study the influence of explant density and subculture duration on blackberry ( Rubus spp. cv. ′Thornfree′) and blueberry ( Vaccinium corymbosum L. cv. ′Brigitta′) was investigated. Four initial densities (6.64, 4.43, 3.32, and 2.21 cm² per explant, considering a vessel bas...

To date there is a growth in fresh and processed blackberry consumption and consequently in their cultivation. The increase in blackberry cultivation increases the demand for quality nursery material for new grove planting. Among plant propagation methods, micropropagation is gaining increasing interest because it allows to overcome some of the disadvantages associated with traditional agamic propagation methods, mostly cuttin...

In vitro propagation, is becoming the predominant method for blackberry propagation due to its advantages compared to agamic traditional propagation methods. Synthetic seed technology represents a promising approach to further enhance the productivity of in vitro propagation facilitating the exchange of plant materials among laboratories and contributing to germplasm conservation efforts. This study aimed to establish an optim...

Selenium (Se) is not essential for plant nutrition, however, growing evidence suggests a role of this element against oxidative stress. Here, we investigated the antioxidant effect of Se in an experimental system consisting of suspensions of olive callus cells exposed to hydrogen peroxide. Due to the disruptive capacity of oxidative stress on cytosolic Ca 2+ homeostasis, Se treatments were simply and effectively assessed with...

Inhalation of olive pollen ( Olea europaea L.) is one of the main causes of allergy in Mediterranean countries and some areas of North America. The response to allergens consists in the production of inflammatory cytokines which is mediated by the deregulation of Ca 2+ signals. In this study, the biological activity of the material released in olive pollen hydration (PMR) was tested on Ca 2+ cytosolic of PE/CA-PJ15 cells (PJ-1...