Wastewater-based epidemiology offers a complementary approach to clinical case-based surveillance of emergent diseases and can help identify regions with infected people to prioritize clinical surveillance strategies. However, tracking emergent diseases in wastewater requires reliance on novel testing assays with uncertain sensitivity and specificity. Limited pathogen shedding may cause detection to be below the limit of quant...

While oxygen is essential for oxidative phosphorylation, O 2 can form reactive species (ROS) when interacting with electrons of mitochondrial electron transport system. ROS is dependent on O 2 pressure (PO 2 ) and has traditionally been assessed in O 2 saturated media, PO 2 at which mitochondria do not typically function in vivo. Mitochondrial ROS can be significantly elevated by the respiratory complex II substrate succinate,...