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Journal of Fish Biology · 2003 · Vol. 63 · Issue 6 · Wiley
Dechorionation or partial digestion of the egg chorion is necessary for introducing embryonic stem cells into blastulas for chimera production and for harvesting blastulas. Several methods to digest the Nile tilapia Oreochromis niloticus chorion were tried and it was found that the chorions of most clutches of eggs were digested in −1 pronase P6911 in 10% Ca/Mg free HBSS. The chorion of Nile tilapia possesses multiple lamellae...
Journal of Biogeography · 2003 · Vol. 30 · Issue 2 · Wiley
Aim Primary succession in a high arctic environment was examined to determine whether the patterns conform to models based on studies in temperate regions. The location also presented an unique opportunity to examine a well preserved pre‐Little Ice Age plant community and organically enriched palaeosol that are being released from the glacier along with the glacial and glaciofluvial sediments. Locations The main location was t...