Olive flounder artificially infected with lymphocystis disease virus (LCDV) were reared at 10, 20 and 30 °C for 60 days, to compare LCD‐incidence. In the fish reared at 20 °C, lymphocystis cells appeared on the skin and fins at 35 days post‐challenge, and the cumulative LCD‐incidence was 80% at 60 days. High levels of LCDV, with a mean polymerase chain reaction (PCR) titre of 10 6 PCR‐U mg −1 tissue, were detected in the fins...