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mRNA delivered by microneedle patch (MNP) can enable painless delivery, reduced need for healthcare expertise, and improved thermostability. In this study, we investigated formulation and manufacturing approaches for developing MNPs that deliver mRNA-loaded lipid nanoparticles (LNPs) encoding luciferase as a reporter protein during MNP fabrication and storage, including mRNA-LNP concentration, formulation, pH, excipients, and...
Molecular adaptations of the estuarine fish Etroplus suratensis in response to salinity fluctuationsOA Marine
Climate-driven shifts in salinity regimes pose significant challenges to aquatic organisms, particularly those inhabiting dynamic coastal ecosystems. Etroplus suratensis (Pearl spot), a euryhaline cichlid capable of thriving across fresh, brackish, and marine environments, offers an excellent model for understanding molecular strategies underpinning salinity acclimation. This study investigated the transcriptomic reprogramming...