RICHARD CHRISTEN results 16
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Genomics‐informed conservation units reveal spatial variation in climate vulnerability in a migratory birdNARA Subscribed
Identifying genetic conservation units (CUs) in threatened species is critical for the preservation of adaptive capacity and evolutionary potential in the face of climate change. However, delineating CUs in highly mobile species remains a challenge due to high rates of gene flow and genetic signatures of isolation by distance. Even when CUs are delineated in highly mobile species, the CUs often lack key biological information...
Accurately quantifying species’ area requirements is a prerequisite for effective area‐based conservation. This typically involves collecting tracking data on species of interest and then conducting home‐range analyses. Problematically, autocorrelation in tracking data can result in space needs being severely underestimated. Based on the previous work, we hypothesized the magnitude of underestimation varies with body mass, a r...
Large birds travel farther in homogeneous environmentsNARA Subscribed
Aim Animal movement is an important determinant of individual survival, population dynamics and ecosystem structure and function. Nonetheless, it is still unclear how local movements are related to resource availability and the spatial arrangement of resources. Using resident bird species and migratory bird species outside the migratory period, we examined how the distribution of resources affects the movement patterns of both...
Marine protist diversity in E uropean coastal waters and sediments as revealed by high‐throughput sequencingNARA Subscribed
Summary Although protists are critical components of marine ecosystems, they are still poorly characterized. Here we analysed the taxonomic diversity of planktonic and benthic protist communities collected in six distant E uropean coastal sites. Environmental deoxyribonucleic acid ( DNA ) and ribonucleic acid ( RNA ) from three size fractions (pico‐, nano‐ and micro/mesoplankton), as well as from dissolved DNA and surface sedi...
Depicting more accurate pictures of protistan community complexity using pyrosequencing of hypervariable SSU rRNA gene regionsNARA Subscribed
Summary Initial environmental pyrosequencing studies suggested highly complex protistan communities with phylotype richness decisively higher than previously estimated. However, recent studies on individual bacteria or artificial bacterial communities evidenced that pyrosequencing errors may skew our view of the true complexity of microbial communities. We pyrosequenced two diversity markers (hypervariable regions V4 and V9 of...
Multiple marker parallel tag environmental DNA sequencing reveals a highly complex eukaryotic community in marine anoxic waterNARA Subscribed
Sequencing of ribosomal DNA clone libraries amplified from environmental DNA has revolutionized our understanding of microbial eukaryote diversity and ecology. The results of these analyses have shown that protist groups are far more genetically heterogeneous than their morphological diversity suggests. However, the clone library approach is labour‐intensive, relatively expensive, and methodologically biased. Therefore, even t...
Summary Plant residues, mainly made up of cellulose, are the largest fraction of organic carbon material in terrestrial ecosystems. Soil microorganisms are mainly responsible for the transfer of this carbon to the atmosphere, but their contribution is not accurately known. The aim of the present study was to identify bacterial populations that are actively involved in cellulose degradation, using the DNA‐stable isotope probing...
The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteriaNARA Subscribed
Summary The phylogenetic diversity of prokaryotic communities exposed to arid conditions in the hot desert of Tataouine (south Tunisia) was estimated with a combination of a culture and – molecular‐based analysis. Thirty‐one isolates, representative of each dominant morphotypes, were affiliated to Actinobacteria , Firmicutes , Proteobacteria and the CFB group while none related to Archaea . Analysis of 16S rRNA gene libraries...
Retrieval of nearly complete 16S rRNA gene sequences from environmental DNA following 16S rRNA‐based community fingerprintingNARA Subscribed
Summary 16S rRNA‐based fingerprinting techniques allow rapid analyses of overall bacterial community structure but suffer from a lack of phylogenetic information hitherto retrievable from the short 16S rRNA gene sequences obtained from excised bands. An approach is presented that allows nearly complete 16S rRNA gene sequences to be retrieved for abundant components of the bacterial community as obtained by the community finger...
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