Microalgae represent a promising but yet underexplored production platform for biotechnology. The vast majority of studies on recombinant protein expression in algae have been conducted in a single species, the green alga Chlamydomonas reinhardtii . However, due to epigenetic silencing, transgene expression in Chlamydomonas is often inefficient. Here we have investigated parameters that govern efficient transgene expression in...

Chloroplast transformation is employed in basic research and green biotechnology. Using chloroplasts as bioreactors provides several advantages such as solar-powered high transgene expression, easy gene-targeting in the plastid genome by homologous recombination, increased biosafety due to tight transgene containment and the potential of engineering metabolic pathways in operon-like arrangements. These and other features make...