Traditional aquaculture can cause serious environmental pollution. Biofilm and biofloc technology have the potential to limit aquaculture pollution. An outdoor experiment was conducted to evaluate the combined effects of eco-substrates and carbon addition on water quality, fish performance and nutrient budgets in the pond polyculture system. In the treatment group, the total ammonia nitrogen, nitrate nitrogen, nitrite nitrogen...

The effects of various levels of alkalinity stress (0, 18.25, 35.41, 52.53 and 69.74 mmol/L) in Chinese mitten crab (Eriocheir sinensis) were investigated by means of measuring hepatopancreas antioxidant system and serum nonspecific immune system-related indices at 0, 12, 24, 48 and 96 h, hepatopancreas tissue structure at 96 h. The activities of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH) and aspartate amino...

Recently, hypoglycemic drugs belonging to sodium-glucose cotransporter 2 inhibitors (SGLT2i) have generated significant interest due to their clear cardiovascular benefits for heart failure with preserved ejection fraction (HFpEF) since there are no effective drugs that may improve clinical outcomes for these patients over a prolonged period. But, the underlying mechanisms remain unclear, particularly its effects on ferroptosi...

Trophic research is essential to the conservation and management of fishery resources. This study analyzed the feeding habits and nutritional interactions among three tuna species in the waters of the Solomon Islands (5°12′S–15°20′S, 157°31′E–172°19′E). A total of 103 bigeye tuna ( Thunnus obesus ), 296 yellowfin tuna ( Thunnus albacores ), and 264 albacore tuna ( Thunnus alalunga ) samples were collected from September to Dec...

Mitten crab, Eriocheir sensu stricto, is an important indigenous aquatic species and food source in Eastern Asia. Genetic diversity is an important prerequisite for the conservation and utilization of germplasm resources. However, the genetic diversity and differentiation of Eriocheir s. s. remains unclear. This study evaluated population genetic diversity and genetic differentiation of three Eriocheir s. s. populations from S...

Studies have indicated that RIG-I may act as a tumor suppressor and participate in the tumorigenesis of some malignant diseases. However, RIG-I induces distinct cellular responses via different downstream signaling pathways depending on the cell type. To investigate the biological function and underlying molecular mechanism of RIG-I in the tumorigenesis of melanoma, we constructed RIG-I knockout, RIG-I -overexpressing B16-F10...

Genetic manipulation of Haematococcus pluvialis is difficult because of the lack of a stable and convenient transformation system. The pH124-EGFP-Ble vector containing ble as a selective gene and EGFP as a reporter gene was constructed and employed for effective transformation. H. pluvialis protoplasts were obtained by treating with cellulase and macerozeme. Then polyethylene glycol-mediated transformation was established by i...