Mass stock enhancement and release are excellent ways to recover Coilia nasus resources. However, it is challenging to evaluate stock enhancement effectively, and it is important to establish a method suitable for estimating C. nasus populations. We explored the effectiveness of marking otoliths in these fish with strontium by immersing C. nasus in hexahydrate strontium chloride solutions. We used laser ablation inductively co...

In order to scientifically and reasonably evaluate the safety risk of ship navigation, to and better solve the problems of the poor sensitivity of static evaluation and insufficient ability to grasp the overall dynamic situation, in this paper, representative safety evaluation indexes for ship navigation are screened and the initial weight of each index is confirmed in combination with the improved analytic hierarchy process (...

Rock joint surface roughness is usually characterized by heterogeneity, but the determination of a required number of samples for achieving a reasonable heterogeneity assessment remains a challenge. In this paper, a novel method, the global search method, was proposed to investigate the heterogeneity of rock joint roughness. In this method, the roughness heterogeneity was characterized based on a statistical analysis of the ro...

Genetic manipulation of Haematococcus pluvialis is difficult because of the lack of a stable and convenient transformation system. The pH124-EGFP-Ble vector containing ble as a selective gene and EGFP as a reporter gene was constructed and employed for effective transformation. H. pluvialis protoplasts were obtained by treating with cellulase and macerozeme. Then polyethylene glycol-mediated transformation was established by i...

Horizontally transferred genes (HTGs) play a key role in animal symbiosis, and some horizontally transferred genes or proteins are highly expressed in specialized host cells (bacteriocytes). However, it is not clear how HTGs are regulated, but microRNAs (miRNAs) are prime candidates given their previously demonstrated roles in symbiosis and impacts on the expression of host genes. A horizontally acquired PanBC that is highly e...

Scatter‐ and larder hoarding are the primary strategies of food‐hoarding animals and have important implications for plant‐animal interactions and plant recruitment. However, their origins and influencing factors have not been fully investigated across a wide range of taxa. Our systematic literature search amassed data for 183 seed‐hoarding rodent species worldwide and tested relationships of seed‐hoarding behaviours with phyl...

As a member of Planctomycetes – Verrucomicrobia – Chlamydia (PVC) superphylum, the phylum Lentisphaerae is broadly distributed in various environments. Lentisphaerae members have been recognized as being typically difficult to isolate in pure culture—only five strains belonging to this phylum were cultured up to now—and therefore their major physiology and ecology are largely unknown. Here, using a polysaccharide degradation-d...