Main conclusion Trait development for commercial heirloom tomatoes can be advanced by optimization of tissue culture and transformation via Agrobacterium and CRISPR/Cas9 mutagenesis. Abstract Genetic improvement using new genome editing approaches relies on the efficient delivery of the CRISPR/Cas system in the vegetable crop tomato. Previous protocols for tomato transformation have primarily focused on a handful of cultivars...

Yellow catfish (Pelteobagrus fulvidraco) is an important freshwater fish species in aquaculture. However, as intensive farming has rapidly expanded, infectious diseases caused by various bacteria, such as Aeromonas hydrophila, have also increased. We conducted a transcriptomic analysis of head kidney from P. fulvidraco stimulated by A. hydrophila strain SHOU. A total of 43,249 unigenes with an average length of 1342 bp were ob...

Intelectins (ITLNs) are a family of calcium-dependent lectins with carbohydrate-binding capacity, are distributed across various vertebrates, and play an important role in the innate immune response against pathogen infection. The yellow catfish Pelteobagrus fulvidraco (Siluriformes: Bagridae) is an economically important fish in China. The aim of this study was to quantify the gene expression of ITLN in response to pathogen-a...