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Journal of Fish Diseases · 2012 · Vol. 35 · Issue 10 · Wiley
A second type of freshwater crab reovirus has been isolated from Chinese mitten crab, Eriocheir sinensis H. Milne Edwards, in China; we named it E. sinensis reovirus ( Es RV816). The negatively stained virion is a non‐enveloped icosahedral particle, 60 ± 5 nm in diameter. Its genome is composed of 10 dsRNA linear pieces exhibiting an electrophoretic pattern of 5/3/2. The largest segment (RNA‐1) was cloned and sequenced. The de...
Journal of Fish Biology · 2012 · Vol. 81 · Issue 1 · Wiley
In this study, complementary DNA (cDNA) and DNA sequences of major histocompatibility complex (MHC) class IIB genes ( mhcIIB ) were cloned from orange‐spotted grouper Epinephelus coioides . The gene structure of E. coioides mhcIIB consists of five exons and four introns, and its deduced amino acid sequence length is 249 amino acids, including a signal peptide, a peptide‐binding region, an IGC1 domain, a transmembrane region an...
Molecular Ecology · 2012 · Vol. 21 · Issue 9 · Wiley
In this study, we explore the population genetics of the Russian wheat aphid (RWA) ( Diuraphis noxia ), one of the world’s most invasive agricultural pests, in north‐western China. We have analysed the data of 10 microsatellite loci and mitochondrial sequences from 27 populations sampled over 2 years in China. The results confirm that the RWAs are holocyclic in China with high genetic diversity indicating widespread sexual rep...