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Journal of Fish Diseases · 2015 · Vol. 38 · Issue 6 · Wiley
A new cell line named CCF ‐ K 104 predominantly consisting of fibroblastic cells showed optimal growth at temperatures from 25 °C to 30 °C. Serial morphological changes in the cells induced by Cyprinid herpesvirus 3 ( C y HV ‐3) included cytoplasmic vacuolar formation, cell rounding and detachment. Mature virions were purified from C y HV ‐3‐infected CCF ‐ K 104 cells by sucrose gradient ultracentrifugation and had a typical h...