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We describe a human and large animal Langendorff experimental apparatus for live electrophysiological studies and measure the electrophysiological changes due to gap junction uncoupling in human and porcine hearts. The resultant ex vivo intact human and porcine model can bridge the translational gap between smaller simple laboratory models and clinical research. In particular, electrophysiological models would benefit from the...
Introduced aquatic macrophytes can dominate small agricultural waterways in summer and autumn becoming a significant management problem. Excessive growth can clog waterways, causing drainage issues and reducing agricultural productivity while in‐stream velocities are reduced and sedimentation increased. Consequently, water managers remove them by mechanical clearance, chemical spray and cutting which can be costly and have neg...
Changes in stream food‐web structure across a gradient of acid mine drainage increase local community stabilityNARA Subscribed
Understanding what makes food webs stable has long been a goal of ecologists. Topological structure and the distribution and magnitude of interaction strengths in food webs have been shown to confer important stabilizing properties. However, our understanding of how variable species interactions affect food‐web structure and stability is still in its infancy. Anthropogenic stress, such as acid mine drainage, is likely to place...
Recent studies have suggested that poikilothermic animals, such as fish, may represent a previously overlooked source of the faecal indicator bacteria (FIB) used for the assessment of water quality. However, quantitative studies of FIB in poikilotherms are scarce. We investigated the presence of FIB in the faeces of five freshwater fish species. E. coli and enterococci were detected in 71 and 76% of faecal samples, respectivel...
Evaluating practical macrophyte control tools on small agricultural waterways in Canterbury, New ZealandNARA Subscribed
Excessive macrophytes can cause significant problems in agricultural waterways requiring active management. Conventional control techniques can have a range of adverse effects. We investigated several control tools in two experiments: firstly, we tested eight treatments at a small‐scale (2 m × 2 m). We found intensive hand weeding, weed mat and herbicide spraying to be effective treatments, reducing macrophyte cover to
Fishes in a changing world: learning from the past to promote sustainability of fish populationsNARA Subscribed
Populations of fishes provide valuable services for billions of people, but face diverse and interacting threats that jeopardize their sustainability. Human population growth and intensifying resource use for food, water, energy and goods are compromising fish populations through a variety of mechanisms, including overfishing, habitat degradation and declines in water quality. The important challenges raised by these issues ha...
Arsenic, often found in gold deposits, can enter aquatic systems. The toxicity of arsenate (As(V)), the most common valence state of arsenic in freshwater, was assessed on the larvae of four freshwater insect taxa from naturally acidic (pH 5.9) and circumneutral (pH 7.4) New Zealand streams. Insects from the acidic stream were more sensitive to arsenate‐spiked water than those from the circumneutral stream. Early instars ( Del...
Comparing total body lipid content of young‐of‐the‐year Steller sea lions among regions of contrasting population trendsNARA Subscribed
Steller sea lion ( Eumetopias jubatus ) young‐of‐the‐year ( YOY ) are nutritionally dependent upon their dam through the majority of their first year. Several indices of body condition were measured in YOY 1.5–9 mo of age captured in Southeast Alaska ( n = 122), the Gulf of Alaska ( n = 182), and the Aleutian Islands ( n = 38) to test the hypothesis that nutritional stress impacted the ability of adult female Steller sea lions...
Summary The anaerobic oxidation of methane (AOM) is mediated by consortia of anaerobic methane‐oxidizing archaea (ANME) and their specific partner bacteria. In thermophilic AOM consortia enriched from Guaymas Basin, members of the ANME‐1 clade are associated with bacteria of the HotSeep‐1 cluster, which likely perform direct electron exchange via nanowires. The partner bacterium was enriched with hydrogen as sole electron dono...