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A quantitative rapid detection method based on loop‐mediated isothermal amplification has been developed for red‐spotted grouper nervous necrosis virus ( RGNNV ). The nested polymerase chain reaction ( PCR ) assay is the mainstream inspection of the brooder in the hatchery. In this study, a real‐time loop‐mediated isothermal amplification ( LAMP ) method has been applied for RGNNV detection, known as a high‐speed gene amplific...
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