A previous proteomic study examining the plasma acute‐phase response of rainbow trout to sterile inflammation highlighted an unidentified 9.5‐kDa spot using 2D‐PAGE, which was dramatically increased. The 15 amino acid sequence obtained from this protein spot allowed rapid amplification of cDNA ends PCR to generate a 443‐bp nucleotide sequence that was 98.6% similar to type‐4 ice‐structuring protein LS‐12 from Atlantic salmon S...

Summary Two prominent characteristics of marine coccolithophores are their secretion of coccoliths and their susceptibility to infection by coccolithoviruses (EhVs), both of which display variation among cells in culture and in natural populations. We examined the impact of calcification on infection by challenging a variety of Emiliania huxleyi strains at different calcification states with EhVs of different virulence. Reduce...

Flavobacterium psychrophilum, the causative agent of bacterial cold‐water disease (BCWD) in freshwater‐reared salmonids, is also a common commensal organism of healthy fish. The virulence potential of F. psychrophilum isolates obtained from BCWD cases in Ontario between 1994 and 2009 was evaluated. In preliminary infection trials of rainbow trout juveniles, significant differences (0% to 63% mortality) in the virulence of the...

Summary Coccolithoviruses employ a suite of glycosphingolipids ( GSLs ) to successfully infect the globally important coccolithophore E miliania huxleyi . Lipid rafts, chemically distinct membrane lipid microdomains that are enriched in GSLs and are involved in sensing extracellular stimuli and activating signalling cascades through protein–protein interactions, likely play a fundamental role in host–virus interactions. Using...

The effects of the 2003 European heatwave on the sea surface layer of the Central Mediterranean were studied using a regional 3-D ocean model. The model was used to simulate the period 2000 to 2004 and its performance was validated using remotely-sensed and in situ data. Analysis of the results focused on changes in the Sea Surface Temperature (SST) and on changes to the surface and sub-surface current field. This permitted us...

The Sicily Channel Regional Model forecasting system was tested using an optimization package for the initial and lateral boundary conditions. Spurious high frequency oscillations during the spin-up time were successfully reduced both in duration and magnitude by optimizing the time tendency of the free surface elevation using the Variational Initialization and Forcing Platform method developed in the framework of the Mediterr...