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Inter-laboratory automation of the in vitro micronucleus assay using imaging flow cytometry and deep learningNARA Subscribed
The in vitro micronucleus assay is a globally significant method for DNA damage quantification used for regulatory compound safety testing in addition to inter-individual monitoring of environmental, lifestyle and occupational factors. However, it relies on time-consuming and user-subjective manual scoring. Here we show that imaging flow cytometry and deep learning image classification represents a capable platform for automat...
This paper surveys the current state of knowledge regarding large-scale meteorological patterns (LSMPs) associated with short-duration (less than 1 week) extreme precipitation events over North America. In contrast to teleconnections, which are typically defined based on the characteristic spatial variations of a meteorological field or on the remote circulation response to a known forcing, LSMPs are defined relative to the oc...
Workers' sons rescue genetic diversity at the sex locus in an invasive honey bee populationNARA Subscribed
The hallmark of eusociality is the division of labour between reproductive (queen) and nonreproductive (worker) females. Yet in many eusocial insects, workers retain the ability to produce haploid male offspring from unfertilized eggs. The reproductive potential of workers has well‐documented consequences for the structure and function of insect colonies, but its implications at the population level are less often considered....
Non‐native acylated homoserine lactones reveal that LuxIR quorum sensing promotes symbiont stabilityNARA Subscribed
Summary Quorum sensing, a group behaviour coordinated by a diffusible pheromone signal and a cognate receptor, is typical of bacteria that form symbioses with plants and animals. LuxIR ‐type N ‐acyl L‐homoserine ( AHL ) quorum sensing is common in Gram‐negative Proteobacteria , and many members of this group have additional quorum‐sensing networks. The bioluminescent symbiont V ibrio fischeri encodes two AHL signal synthases:...
Summary This study reports the first description and molecular characterization of naturally occurring, non‐bioluminescent strains of Vibrio fischeri . These ‘dark’ V. fischeri strains remained non‐bioluminescent even after treatment with both autoinducer and aldehyde, substrate additions that typically maximize light production in dim strains of luminous bacteria. Surprisingly, the entire lux locus (eight genes) was absent in...
Summary Recent evidence has indicated that natural genetic transformation occurs in Vibrio cholerae, and that it requires both induction by chitin oligosaccharides, like chitohexaose, and expression of a putative regulatory gene designated tfoX . Using sequence and phylogenetic analyses we have found two tfoX paralogues in all sequenced genomes of the genus Vibrio . Like V. cholerae , when grown in chitohexaose, cells of V. fi...