Peanut is a major oilseed crop in the U.S., which ranks third globally in production, with South Carolina ranking sixth nationally. Despite their economic and nutritional value, peanuts are unsuitable for 1–2% of the U.S. population due to allergic reaction, including anaphylaxis, making the development of reduced-allergen peanuts a priority. This study aimed to identify peanut lines with reduced Ara h1, h2, h3, and h6 levels...