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Haploid embryogenic cultures of maize (Zea mays L.): stability, doubling efficiency, and use in gene editingNARA Subscribed
Embryogenic cultures were initiated from haploid and diploid seedlings of maize inbred LH244 and monitored for ploidy stability by flow cytometry over the course of six mo. Both culture types were transformed with three different constructs using Agrobacterium tumefaciens to compare transformation and gene editing frequencies. Paromomycin resistant cell lines were split prior to plant regeneration, and half of the tissue was t...
Marine megafauna species are affected by a wide range of anthropogenic threats. To evaluate the risk of such threats, species’ vulnerability to each threat must first be determined. We build on the existing threats classification scheme and ranking system of the International Union for Conservation of Nature (IUCN) Red List of Threatened Species by assessing the vulnerability of 256 marine megafauna species to 23 at‐sea threat...
Air-template assisted enhancement of solar steam generationNARA Subscribed