Background and aims Microbes in the rhizosphere play a crucial role in regulating various agroecosystem functions that determine crop productivity. While management practices influence the structure and composition of these microbial communities, our understanding of this influence in farmers' fields remains limited at regional scale. Methods In this study, we conducted an on-farm study to investigate the rhizosphere microbiom...

Protists, a crucial part of the soil food web, are increasingly acknowledged as significant influencers of nutrient cycling and plant performance in farmlands. While topographical and climatic factors are often considered to drive microbial communities on a continental scale, higher trophic levels like heterotrophic protists also rely on their food sources. In this context, bacterivores have received more attention than fungiv...

Organic carbon and aggregate stability are key features of soil quality and are important to consider when evaluating the potential of agricultural soils as carbon sinks. However, we lack a comprehensive understanding of how soil organic carbon (SOC) and aggregate stability respond to agricultural management across wide environmental gradients. Here, we assessed the impact of climatic factors, soil properties and agricultural...

Summary Composting is widely used to reduce the abundance of antibiotic resistance genes (ARGs) in solid waste. While ARG dynamics have been extensively investigated during composting, the fate and abundance of residual ARGs during the storage remain unexplored. Here, we tested experimentally how ARG and mobile genetic element (MGE) abundances change during compost storage using metagenomics, quantitative PCR and direct cultur...

Summary Land‐use change is one of the most important factors influencing soil microbial communities, which play a pivotal role in most biogeochemical and ecological processes. Using agroforestry systems as a model, this study examined the effects of land uses and edaphic properties on bacterial communities in three agroforestry types covering a 270 km soil‐climate gradient in A lberta, C anada. Our results demonstrate that lan...

Summary Soil denitrification is one of the most significant contributors to global nitrous oxide (N 2 O) emissions, and spatial patterns of denitrifying communities and their functions may reveal the factors that drive denitrification potential and functional consortia. Although denitrifier spatial patterns have been studied extensively in most soil ecosystems, little is known about these processes in arctic soils. This study...